Detection of DNA Markers in Dogs with Patellar Luxation by High Annealing Temperature - Random Amplified Polymorphic DNA Analysis
نویسندگان
چکیده
منابع مشابه
Genetic analysis with random amplified polymorphic DNA markers.
For many years, the principles of genetics have been applied to crop variety improvement with great success. Severa1 crop species, notably corn, wheat, and tomato, have been used as model genetic systems because of their central importance to food production. Until recently, virtually a11 progress in both breeding and model genetic systems has relied on a phenotypic assay of genotype. Because t...
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Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
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Cashmere is only produced from Maraz (Kurdi) goat breed in Iraqi Kurdistan Region. The objective of this study was to assess the genetic diversity of different Maraz color types, black goat and Shami breeds using random amplified polymorphic DNA (RAPD) markers. Fourteen primers were used and nine out of them were selected based on their number of bands (NB) and polymorphic characteristics. Thes...
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In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...
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Random-amplified polymorphic DNA (RAPD) markers (8) are a powerful tool for genome analysis (7). Nevertheless, the difficulties to achieve a high pattern repeatability represent a major drawback for the routine implementation of RAPD markers. Amplification products obtained depend on various factors, such as the concentration of the reaction mixture constituents, the source of Taq DNA polymeras...
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ژورنال
عنوان ژورنال: Kafkas Universitesi Veteriner Fakultesi Dergisi
سال: 2014
ISSN: 1300-6045
DOI: 10.9775/kvfd.2013.9888